한국인 구강 편평세포암종에서의 Ha-ras gene의 점돌연변이(point mutation)에 관한 연구
Ha-ras gene point mutation in the oral squamous cell carcinoma of Korean
오성섭, 박시훈,
소속 상세정보
오성섭 ( ) - 연세대학교 식품공학과
박시훈 ( ) - 연세대학교
KMID : 0355619940200030273
Abstract
The possible causes of oral squamous cell carcinoma include smoking, alcohol, viruses and chemicals. About 24 oncogenes involving the Ha-ras gene are known to be related to human and animal cancer. The ras gene point mutation varies among
different
tumors; for examples, Ha-ras gene is more frequent in human bladder carcinomas, Ki-ras gene is more frequent in lung and colon carcinomas, and N-ras gene in hematological tumors. The Ha-ras gene point mutation of oral SCC(squamous cell carcinoma)
is
variant from the race and geography, it appears to be frequent(about 35%) in Indian, but appears to be rare(about 3%) in Western.
This study was undertaken to investigate the rate of Ha-ras gene point mutation of the oral SCC in Korean. DNA extraction, PCR and SSCP, DNA sequencing of Ha-ras gene were done, and the point mutation was searched in the formaline fixed,
paraffin-embeded tissues of the oral SCC from the 39 Korean patients (M:29, F:10).
@ES Results were as follows:
@EN 1. The exon 1 of Ha-ras gene of 39 oral SCC was found in the 3 types(A, B, Ctypes) of band shift in the SSCP.
Each SSCP type A & B (37cases) of exon 1 has a normal DNA sequence, and no point mutation was detected.
Confirmation of SSCP type C of exon 1 was not possible presumably because of the artifact associated with DNA template purities.
The exon 2 of the Ha-ras gene in 39 ora SCC was found as a regular type of band shift in SSCP universally.
From these results, the point mutation of the Ha-ras gene of oral SCC in Korean proved to be rare, and other oncogenes than the Ha-ras gene may be related to carcinogenesis of oral SCC. Verifying the other oncogenes thought to be quite helpful in
the
treatment, prediagnosis, carcinogenesis of oral cancer.
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